Top Guidelines Of thr77
Top Guidelines Of thr77
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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has been noticed in many tumors, which results in chromosome instability, and thus may possibly contribute to tumorigenesis. This protein was found to associate with, and be associated with, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle regulated histone gene expression and performs a critical function in selling cell-cycle progression while in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinctive isoforms, are described. Two supplemental splice variants have been documented but specific nucleotide sequence info is not really nonetheless accessible.
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Blocking peptides are peptides that bind precisely towards the target antibody and block antibody binding. These peptide normally is made up of the epitope regarded because of the antibody.
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This information outlines further doable optimization of mobile and immunoassay parameters to be sure the very best final results are...
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This guide outlines more probable optimization of mobile and immunoassay parameters to ensure the best possible success are...
Antibodies certain to the blocking peptide no more bind to the epitope about the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is unique.
Goal Information Cyclin E belongs on the highly conserved cyclin household, whose users are characterized by a spectacular periodicity in protein abundance with the mobile cycle. Cyclins perform as regulators of CDK kinases. Unique cyclins show distinct expression and degradation styles which lead towards the temporal coordination of each and every mitotic celebration. Cyclin E kinds a complex with and capabilities being a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S transition. This protein accumulates within the G1-S phase boundary and is degraded as cells progress as a result of S period.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both antibodies...
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